Thermo Fisher Scientific Mouse anti-Rat IgG2a Secondary Antibody, FITC

Applications

Tested Dilution

Flow Cytometry (Flow)

5 µg/mL

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Secondary Antibody

Clone

MARG2a-1

Immunogen

Rat IgG2a

Conjugate

FITC FITC FITC

View additional formats

• [eFluor 570](/antibody/secondary/product-name/Rat IgG2a/conjugates/eFluor 570/host/Mouse/)
• [eFluor 615](/antibody/secondary/product-name/Rat IgG2a/conjugates/eFluor 615/host/Mouse/)
• [eFluor 660](/antibody/secondary/product-name/Rat IgG2a/conjugates/eFluor 660/host/Mouse/)
• [PE ](/antibody/secondary/product-name/Rat IgG2a/conjugates/PE/host/Mouse/)
• [PerCP-eFluor 710](/antibody/secondary/product-name/Rat IgG2a/conjugates/PerCP-eFluor 710/host/Mouse/)
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_794591

Target

IgG2a

Antibody Form

Whole Antibody

Product Specific Information

SA1-25265 detects rat IgG2a, no cross reactivity is observed with other rat immunoglobulin subclasses.

Use 10 µL of the suggested dilution to label 10^6 cells in 100 µL.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.