Thermo Fisher Scientific eEF1G Polyclonal Antibody
Rabbit 폴리클로날 항체로 인간 EEF1G 단백질(잔기 200–250)을 인식합니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 액상 형태로 4°C에서 보관합니다. 연구용으로만 사용되며 FFPE 조직에서는 Tris-EDTA pH 9.0 전처리를 권장합니다.
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Thermo Fisher Scientific eEF1G Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 200 and 250 of human Eukaryotic Translation Elongation Factor 1 Gamma (EEF1G, UniProt ID: P26641-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
Target Information
EF-1 (elongation factor-1) is a multi-protein complex responsible for delivering aminoacyl-tRNAs to the ribosome. EF-1 gamma (EEF1G or GIG35) is a 437 amino acid subunit of the EF-1 complex. It is expressed in stomach, pancreas, brain, lung, kidney, intestine, liver, and spleen. EF-1 gamma contains an N-terminal Glutathione transferase domain that anchors the complex to cellular components and may be involved in assembling multiprotein complexes containing aminoacyl-tRNA synthetases. Increased expression of EF-1 gamma is associated with pancreatic cancer, suggesting a potential role in oncogenic transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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