Thermo Fisher Scientific S100A4/Metastasin/Calvasculin Recombinant Rabbit Monoclonal Antibody (S100A4, 2750R)
S100A4 단백질을 인식하는 토끼 단클론 항체로, 인간 및 마우스 시료에 반응합니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. HEK293 세포에서 발현된 재조합 항체이며, 보존제가 없는 PBS 용액 형태로 제공됩니다.
- 카탈로그번호
- 6275-RBM4-P1ABX
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | S100A4, 2750R |
| Immunogen | Recombinant fragment of human S100A4 protein (around aa1–200) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For immunohistochemistry (PFA-fixed samples), incubate the antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The S100A4 gene encodes a member of the S100 family of calcium-binding proteins containing two EF-hand motifs.
S100 proteins are localized in the cytoplasm and/or nucleus of various cell types and regulate multiple cellular processes such as cell cycle progression and differentiation.
S100 genes are clustered on chromosome 1q21, and S100A4 is associated with cell motility, invasion, and tubulin polymerization.
Altered expression or chromosomal rearrangements of this gene have been linked to tumor metastasis.
Multiple alternatively spliced variants encoding the same protein have been identified.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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