
Thermo Fisher Scientific PRAK Polyclonal Antibody
PRAK 단백질을 인식하는 염소 폴리클로날 항체로 Western blot, IHC(P), IP에 사용 가능. Human 및 Mouse 반응성. 0.5 mg/mL 농도의 액상 형태로 제공되며, TBS(pH 7.3) 및 0.5% BSA 포함. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 4–8 µg/mL | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence C-STEALDNVLPSAQ |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2933646 |
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
Target Information
MAPKAPK5 (PRAK) is activated in response to cellular stress and pro-inflammatory cytokines. Activity of this kinase is regulated by p38 alpha and p38 beta. The protein encoded by PRAK is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of PRAK encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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