Thermo Fisher Scientific CKMT2 Polyclonal Antibody
Rabbit polyclonal antibody targeting human CKMT2, validated for WB, IHC(P), and ICC/IF. Recombinant fragment immunogen (aa 1–224). High specificity and affinity-purified. Suitable for mitochondrial creatine kinase research. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 1–224 of Human CKMT2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546067 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2
- Predicted reactivity: Mouse (91%), Rat (91%), Dog (94%), Pig (94%), Rabbit (92%), Chicken (84%), Bovine (92%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Mitochondrial creatine kinase (MtCK) mediates the transfer of high-energy phosphate from mitochondria to cytosolic creatine. It belongs to the creatine kinase isoenzyme family, existing as sarcomeric and ubiquitous isoenzymes encoded by separate genes. MtCK occurs in dimeric and octameric forms, unlike the exclusively dimeric cytosolic isoenzymes. Sarcomeric MtCK shares 80% homology with ubiquitous MtCK. This gene includes sequences homologous to motifs found in nuclear genes encoding mitochondrial proteins, potentially crucial for coordinated activation during mitochondrial biogenesis. Three transcript variants encoding the same protein have been identified.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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