Thermo Fisher Scientific IL-4 Monoclonal Antibody (25D2), Biotin
인간 IL-4를 특이적으로 인식하는 rat monoclonal antibody로 Biotin 결합형 제품입니다. WB, ELISA 등 다양한 응용에 적합하며, recombinant human IL-4를 면역원으로 사용했습니다. 고순도 Protein G 정제 및 안정적인 PBS/BSA buffer 포뮬레이션을 제공합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| ELISA | 0.003–12.5 µg/mL | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 25D2 |
| Immunogen | Recombinant human IL-4 (CHO cell-derived) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 4% BSA |
| Contains | Proprietary preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_223569 |
Related Formats
Product Specific Information
M450B targets IL-4 in WB and ELISA applications and shows reactivity with Human samples.
The M450B immunogen is recombinant human IL-4 (CHO cell-derived).
M450B detects IL-4, which has a predicted molecular weight of approximately 15 kDa.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the interleukin 4 receptor. IL-4 has a broad range of actions. The interleukin 4 receptor also binds to IL13, contributing to overlapping functions of these cytokines.
STAT6 plays an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, along with IL-3, IL-5, IL-13, and CSF2, forms a cytokine gene cluster on chromosome 5q. IL-4 is located near IL13, and both genes are coordinated by long-range regulatory elements over a 120 kb range. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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