
Thermo Fisher Scientific 4-1BB Ligand Recombinant Rabbit Monoclonal Antibody (014)
Mouse TNFSF9/4-1BBL 특이성을 가진 Thermo Fisher recombinant rabbit monoclonal antibody. 다양한 응용(WB, ICC/IF, Flow, ELISA)에 사용 가능. 보존제 무첨가, 높은 특이성과 재현성 제공. 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:1,000 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| ELISA | 1:1,000–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 014 |
| Immunogen | Recombinant Mouse TNFSF9 protein (Arg104–Glu309) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785657 |
Additional Formats
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to avoid contamination.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody has specificity for Mouse TNFSF9/4-1BBL.
Target Information
CD137 ligand (CD137L) is a type II membrane protein and member of the TNF superfamily. It acts as a co-stimulatory molecule expressed on antigen-presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137/4-1BB, is found on various cells, including inflamed endothelial cells, enhancing monocyte extravasation.
Activated T cells express CD137, and engagement with CD137L promotes:
- T cell proliferation
- IL-2 secretion
- Survival and cytotoxic activity
CD137L is expressed in most B-cell lymphomas (mantle cell, follicular, diffuse large B-cell) and carcinoma cell lines, but absent in Hodgkin and T-cell lymphomas. It is implicated in T cell–tumor cell interactions.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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