Thermo Fisher Scientific SPHK1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 321 and 384 of Human SPHK1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SPHK1,uniProtId:Q9NYA1-1,ncbiNodeId:9606,antigenRange:321-384,antigenLength:384,antigenImageFileName:PA5-28099_SPHK1_Q9NYA1-1_Rabbit.svg,antigenImageFileNamePDP:PA5-28099_SPHK1_Q9NYA1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545575

Product Specific Information

Recommended positive controls: A431, Molt-4.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK1 catalyzes the phosphorylation of sphingosine to sphingosine 1 phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.