Thermo Fisher Scientific SPHK1 Polyclonal Antibody
SPHK1 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF에 사용 가능. Human에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS(glycerol 포함) 용액 형태로 제공되며, 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 321–384 of Human SPHK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.17 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545575 |
Product Specific Information
- Recommended positive controls: A431, Molt-4
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P).
S1P signals through G-protein coupled receptors and controls diverse cellular processes.
Inhibitors of SPHKs are viewed as potential therapeutics for cancer treatment.
SPHK1 catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P), which acts as a signaling molecule both intra- and extracellularly.
S1P regulates cell differentiation, calcium mobilization, and apoptosis.
The cell surface receptors for S1P are members of the EDG family of G protein-coupled receptors (S1P Receptors).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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