Thermo Fisher Scientific ATG4B Polyclonal Antibody
ATG4B 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IHC(P)에서 검증됨. Human, Mouse, Rat에 반응하며, 1 mg/mL 농도의 액상 형태로 제공. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 보장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B. The immunogen is located within the last 50 amino acids of ATG4B. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2890417 |
Product Specific Information
Positive controls that may be used include:
- Ramos Cell Lysate
- Jurkat Cell Lysate
- Human Spleen Tissue Lysate
- Human Spleen Tissue Slide
Target Information
Cysteine protease ATG4B is an enzyme coded by the ATG4B gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and proteins. ATG4B acts as a deubiquitin-like protease playing dual roles in the core machinery of autophagy. It cleaves the C-terminal amino acid of ATG8 family proteins (MAP1LC3, GABARAPL1, GABARAPL2, and GABARAP) to reveal a C-terminal glycine. Exposure of the glycine is essential for conjugation of ATG8 proteins to phosphatidylethanolamine (PE) and membrane insertion, which are necessary for autophagy. ATG4B is mainly expressed in skeletal muscle, brain, heart, liver, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
- PA5-114497_ATG4B_Q9Y4P1-1_Rabbit.svg
- PA5-114497_ATG4B_Q9Y4P1-1_Rabbit_PDP.jpeg
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