Thermo Fisher Scientific UCP3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

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Immunohistochemistry (Paraffin) (IHC (P))

1:3,000

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, conjugated to KLH, corresponding to residues 254-267 of human UCP3 with an N-terminal added lysine. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:UCP3,uniProtId:P55916-1,ncbiNodeId:9606,antigenRange:254-267,antigenLength:312,antigenImageFileName:PA1-24895_UCP3_P55916-1_Rabbit.svg,antigenImageFileNamePDP:PA1-24895_UCP3_P55916-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2257029

Product Specific Information

Recommended positive controls: human and mouse skeletal muscle, mitochondrial extract of rat brown adipose tissue.

The corresponding sequence in rat and mouse UCP3 differ by 4 and 3 amino acids respectively. This antibody exhibits no cross reactivity with UCP1 or UCP2.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The uncoupling protein UCP1 (formerly designated UCP) is an integral membrane protein unique to brown adipose tissue mitochondria. UCP1 forms a dimer that acts as a proton channel, which can uncouple oxidative phosphorylation by dissipating the electrochemical potential across the inner mitochondrial membrane. This process induces heat production in brown adipose tissue and is involved in regulation of body temperature and glucose metabolism. UCP2 is a structurally related protein that also uncouples mitochondrial respiration. It is more widely expressed in human and mouse tissues, including white adipose tissue and muscle, than is UCP1. UCP2 is thought to play a role in body weight regulation. An additional UCP family member, UCP3, is highly muscle specific and is possibly involved in the uncoupling of oxidative phosphorylation in skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.