Thermo Fisher Scientific UCP3 Polyclonal Antibody
UCP3 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P) 등에 적합합니다. Human, Mouse, Rat 시료에 반응하며 UCP1, UCP2와 교차 반응하지 않습니다. 항원 친화 크로마토그래피로 정제되었으며, PBS(pH 7.4) + 1% BSA 용액에 보존됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:3,000 | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to residues 254–267 of human UCP3 with an N-terminal added lysine |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 15 mM sodium azide |
| Storage conditions | Store at 4°C (short term); for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2257029 |
Product Specific Information
- Recommended positive controls: human and mouse skeletal muscle, mitochondrial extract of rat brown adipose tissue
- The corresponding sequence in rat and mouse UCP3 differ by 4 and 3 amino acids respectively
- No cross-reactivity with UCP1 or UCP2
- Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
Target Information
The uncoupling protein UCP1 (formerly designated UCP) is an integral membrane protein unique to brown adipose tissue mitochondria. UCP1 forms a dimer that acts as a proton channel, uncoupling oxidative phosphorylation by dissipating the electrochemical potential across the inner mitochondrial membrane. This process induces heat production in brown adipose tissue and is involved in regulation of body temperature and glucose metabolism.
UCP2 is a structurally related protein that also uncouples mitochondrial respiration and is more widely expressed in human and mouse tissues, including white adipose tissue and muscle. It is thought to play a role in body weight regulation.
UCP3, another UCP family member, is highly muscle specific and may be involved in the uncoupling of oxidative phosphorylation in skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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