Thermo Fisher Scientific Dicer Monoclonal Antibody (CL0378)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

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Immunohistochemistry (IHC)

-

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Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunohistochemistry (Frozen) (IHC (F))

-

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Immunocytochemistry (ICC/IF)

2-10 µg/mL

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

CL0378

Immunogen

Recombinant protein corresponding to Human DICER1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Dicer,uniProtId:Q9UPY3-1,ncbiNodeId:9606,antigenRange:869-1062,antigenLength:1922,antigenImageFileName:MA5-31353_Dicer_Q9UPY3-1_House_mouse.svg,antigenImageFileNamePDP:MA5-31353_Dicer_Q9UPY3-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2786990

Product Specific Information

Immunogen sequence: PTDADSAYCV LPLNVVNDSS TLDIDFKFME DIEKSEARIG IPSTKYTKET PFVFKLEDYQ DAVIIPRYRN FDQPHRFYVA DVYTDLTPLS KFPSPEYETF AEYYKTKYNL DLTNLNQPLL DVDHTSSRLN LLTPRHLNQK GKALPLSSAE KRKAKWESLQ NKQILVPELC AIHPIPASLW RKAVCLPSIL YRLH

Target Information

Dicer is a double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3` overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a means to specifically regulate the expression of target genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.