Thermo Fisher Scientific NF-kB p65 Monoclonal Antibody (3G1)
Rabbit monoclonal antibody recognizing human NF-kB p65 (RelA) for WB, IHC, ICC/IF, and Flow Cytometry. Validated applications with high specificity. Supplied as 1 mg/mL liquid, stored at -20°C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3G1 |
| Immunogen | Human NF-kB p65 between amino acids 500 to the C-terminus |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
Target Information
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene located on chromosome 11 in humans. Also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A), it belongs to the Rel family of proteins. NF-kB p65 is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells), which forms heterodimers with subunits such as p50 or p52. Upon TNF alpha receptor activation, IKK becomes phosphorylated and subsequently phosphorylates I kB, leading to its proteasomal degradation. NF-kB p65 plays a key role in the transcription of the immunoglobulin kappa gene in mature B-lymphoid cells.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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