Thermo Fisher Scientific CD244 Monoclonal Antibody (eBioPP35 (PP35)), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats:

• Unconjugated
• Request custom conjugation

Product Specific Information

The eBioPP35 monoclonal antibody reacts with human CD244 (also known as 2B4, p38, NK-cell activation-inducing ligand, SLAMF4, and natural killer cell receptor 2B4).
CD244 is a 38 kD protein expressed on NK cells, subsets of CD8+ T cells, γδ T cells, monocytes, basophils, mast cells, and eosinophils.
Binding of CD244 to its ligand CD48 results in NK cell activation. Costaining experiments with eBioC1.7 indicate recognition of different epitopes.

Applications Tested:
This eBioPP35 (PP35) antibody has been tested by flow cytometric analysis of human peripheral blood cells.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:
Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level:
Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation:
Less than 10%, as determined by HPLC.

Target Information

CD244 (2B4, SLAMF4) is a cell surface receptor expressed on NK cells and some T cells that mediate non-MHC restricted killing.
Interaction between NK and target cells via CD244 modulates NK-cell cytolytic activity.
CD244 interacts with SLAMF2, activating both SLAMF4- and SLAMF2-expressing cells.
Patients with systemic lupus erythematosus have reduced SLAMF4 expression on NK cells and monocytes, suggesting involvement in autoimmune pathology.
Diseases associated with CD244 dysfunction include rheumatoid arthritis and lymphoproliferative syndrome.
Alternatively spliced transcript variants encoding different isoforms of CD244 have been identified.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.