Thermo Fisher Scientific FDFT1 Polyclonal Antibody
Rabbit polyclonal antibody against human FDFT1 (Squalene synthetase). Validated for WB and IHC(P). High purity via antigen affinity chromatography. Supplied as a liquid at 0.97 mg/mL. For research use only.
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- PA528912
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- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–395 of Human Squalene synthetase (SQS) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.97 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546388 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HepG2, Molt-4, Raji
- Predicted reactivity: Mouse (88%), Rat (86%), Pig (91%), Chicken (80%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (91%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
FDFT1 catalyzes the first step in the cholesterol biosynthetic pathway, converting trans-farnesyldiphosphate to squalene. The loss of promoter activity and response to sterols for FDFT1 is localized to a 69-bp section positioned 131 bp 5′ to the transcription start site. This region contains a sterol regulatory element-1 (SRE1) and two putative NF1 binding sites.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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