Thermo Fisher Scientific Cardiac Troponin C Polyclonal Antibody
Cardiac Troponin C 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot 및 Immunocytochemistry에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full-length Human Cardiac Troponin C (UniProt ID: P63316-1, amino acids 1–161) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2720368 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by antigen affinity chromatography using an epitope-specific immunogen. Purity is greater than 95% as determined by SDS-PAGE.
Target Information
Troponin (Tn) is the central regulatory protein of striated muscle contraction. It consists of three components:
- Tn-I: Inhibitor of actomyosin ATPase
- Tn-T: Contains the binding site for tropomyosin
- Tn-C: Binds calcium ions
The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Calcium-ion uptake by troponin-C induces allosteric changes in the muscle thin filament, enabling actin-myosin interaction, ATP hydrolysis, and tension generation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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