Thermo Fisher Scientific ATG4B Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive controls: HepG2, HCT116
• Predicted reactivity: Human (99%), Mouse (91%), Rat (91%), Pig (91%), Bovine (86%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cysteine protease ATG4B is an enzyme encoded by the ATG4B gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and proteins. ATG4B is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. It cleaves the C-terminal amino acid of ATG8 family proteins (MAP1LC3, GABARAPL1, GABARAPL2, and GABARAP) to reveal a C-terminal glycine. This glycine exposure is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and membrane insertion, which is necessary for autophagy. ATG4B is mainly expressed in skeletal muscle, brain, heart, liver, and pancreas.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.