Thermo Fisher Scientific CENPB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

ChIP assay (ChIP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.10 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2662433

Product Specific Information

Immunogen sequence: TFREKSRIIQE VEENPDLRKG EIARRFNIPP STLSTILKNK RAILASERKY GVASTCRKTN KLSPYDKLEG LLIAWFQQIR AAGLPVKGII L

Highest antigen sequence identity to the following orthologs - mouse 100%, rat 100%.

Target Information

CENPB is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes and is considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.