Thermo Fisher Scientific GFP Polyclonal Antibody, Biotin

Applications and Tested Dilutions

Product Specifications

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.

As a fusion tag, GFP can be used to localize proteins, study their movement, or research the dynamics of subcellular compartments. GFP technology has revealed new insights into the physiological activities of living cells.

GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (maximally at 395 nm) and emits green light (peak emission 508 nm) without requiring additional proteins, substrates, or cofactors. GFP fluorescence is stable, species-independent, and suitable for a wide variety of applications.

GFP has been extensively used as a fluorescent tag to monitor gene expression and protein localization, as well as to assess protein-protein interactions (yeast two-hybrid system) and measure distances between proteins in FRET experiments.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.