Thermo Fisher Scientific IRAK4 Polyclonal Antibody
인간 IRAK4 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunocytochemistry에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며 PBS 버퍼에 0.02% sodium azide를 포함합니다. 연구용으로 NF-κB 및 MAPK 신호전달 연구에 활용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1–4 µg/mL
- Publication: View 1 publication
Immunocytochemistry (ICC/IF)
- Tested Dilution: 5–10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to amino acids at the carboxy terminus of human IRAK-4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152798 |
Product Specific Information
- In Western blot applications, this antibody detects a band at approximately 50 kDa.
- Suggested positive control: K562 cell lysate.
- PA5-20018 can be used with blocking peptide PEP-0137.
Target Information
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways.
Overexpression of IRAK-4 activates NF-kappa-B and MAPK pathways.
IRAK-4 interacts with and phosphorylates IRAK-1.
IRAK-4-deficient animals are completely resistant to challenge with LPS, and both animals and humans lacking IRAK-4 show impaired responses to viral and bacterial challenges.
Members of the IRAK/Pelle family play a central role in IL-1R/TLR-mediated inflammatory responses and innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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