Thermo Fisher Scientific IRAK4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-4 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5-10 µg/mL

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Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152798

Product Specific Information

In Western blot applications, this antibody detects a band at ~50kDa. A suggested positive control is K562 cell lysate.

PA5-20018 can be used with blocking peptide PEP-0137.

Target Information

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. Overexpression of IRAK-4 activates NF-kappa-B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.