Thermo Fisher Scientific TRIB1 Polyclonal Antibody
TRIB1 단백질을 인식하는 Goat Polyclonal 항체로, Western blot에 적합합니다. 0.5 mg/mL 농도의 액상 형태이며, TBS(pH 7.3)와 0.5% BSA, 0.02% sodium azide를 포함합니다. -20°C에서 보관하며 반복 동결-해동은 피해야 합니다.
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Applications
- Western Blot (WB): 0.3–1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence C-KHYQDKIRPYIQLPS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2933153 |
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:64,000.
Target Information
TRIB1 (also known as gprotein coupled receptor induced, skip1, gig2, c8fwATP) functions in protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities.
Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity.
Overexpression of TRIB1 inhibited oncogenic Ras-driven AP1 activation and MEKK1-mediated AP1 activation.
ERK activation was enhanced by TRIB1.
Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1.
Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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