Thermo Fisher Scientific Collagen II Monoclonal Antibody (2B1.5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50

View 10 publications 10 publications

Immunohistochemistry (IHC)

-

View 60 publications 60 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 15 publications 15 publications

Flow Cytometry (Flow)

Assay-dependent

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Published species

Bovine, Goat, Guinea pig, Human, Mouse, Pig, Rabbit, Rat, Sheep

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2B1.5

Immunogen

A purified preparation of lathyritic type II collagen from embryonic chicken sternum if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Collagen II,uniProtId:P02460-1,ncbiNodeId:9031,antigenRange:1-859,antigenLength:859,antigenImageFileName:MA1-37493_Collagen_II_P02460-1_House_mouse.svg,antigenImageFileNamePDP:MA1-37493_Collagen_II_P02460-1_House_mouse_PDP.jpeg,sortOrder:5}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2082337

Product Specific Information

Staining of formalin/parafin tissues requires disgestion of tissue sections with pepsin at 1 mg/mL Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C.

Target Information

COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.