Thermo Fisher Scientific Collagen II Monoclonal Antibody (2B1.5)
Type II collagen에 특이적인 mouse monoclonal antibody로, cartilage 연구 및 질병 관련 단백질 분석에 적합함. Western blot, IHC, ICC 등 다양한 응용 가능. Protein A로 정제된 액상 제품으로 안정적인 성능 제공.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:50 | View 10 publications |
| Immunohistochemistry (IHC) | - | View 60 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 15 publications |
| Flow Cytometry (Flow) | Assay-dependent | View 2 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Chicken, Human, Mouse, Rat |
| Published Species | Bovine, Goat, Guinea pig, Human, Mouse, Pig, Rabbit, Rat, Sheep |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B1.5 |
| Immunogen | Purified preparation of lathyritic type II collagen from embryonic chicken sternum |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2082337 |
Additional Formats:
Product Specific Information
Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1 mg/mL Tris-HCl, pH 2.0 for 15 min at room temperature or 10 min at 37°C.
Target Information
COL2A1 encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye.
Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type.
Defects in processing chondrocalcin, a calcium-binding protein that is the C-propeptide of this collagen molecule, are also linked to chondrodysplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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