Thermo Fisher Scientific PolarScreen Androgen Receptor Competitor Assay Kit, Green

Thermo Fisher Scientific PolarScreen™ Androgen Receptor Competitor Assay Kit, Green

The PolarScreen™ Androgen Receptor Competitor Assay Kit, Green provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential androgen receptor (AR) ligands.
This kit uses insect cell-expressed rat AR-ligand binding domain tagged with glutathione-S-transferase (GST) and histidine, along with a novel, high-affinity fluorescent androgen ligand (Fluormone™ AL Green) in a homogeneous, mix-and-read assay format.

This kit produces a green color read-out; a red version is also available. Both kits determine the IC50 of competitive compounds. Color selection depends on the spectral properties of screened compounds and available instrumentation.

For additional information or assistance, contact: drugdiscoverytech@lifetech.com

주요 특징

• 완전형 키트: 모든 시약 포함, 시험 화합물만 추가하면 바로 사용 가능
• 고처리량 분석 호환: 384웰 형광 편광 실험 가능
• 대량 스크리닝을 위한 벌크 사이즈 구성품 제공

Assay 작동 원리

The kit uses the rat AR ligand-binding domain tagged with His and GST [AR-LBD(His-GST)].
AR-LBD(His-GST) is added to a fluorescently-tagged androgen ligand (Fluormone™ AL Green) in the presence of competitor test compounds in microwell plates.
Effective competitors prevent formation of the AL Green/AR-LBD(His-GST) complex, resulting in decreased polarization value due to ligand displacement.
The shift in polarization value indicates the relative affinity of test compounds for AR-LBD(His-GST).

Contact Thermo Fisher Scientific for bulk quantities or assistance with other nuclear receptor assay needs—from assay development to library screening and beyond.

제품 사양