Thermo Fisher Scientific MCM3 Polyclonal Antibody
MCM3 단백질을 인식하는 Rabbit Polyclonal Antibody로, Human 및 Mouse에 반응합니다. Western blot, IHC, ICC/IF에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 1 mg/mL 농도의 액상 형태로 제공되며, 4°C 또는 -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 42–302 of MCM3 (Uniprot ID: P25205) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | Store at 4°C (short term). For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_11153207 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji
- Predicted reactivity: Mouse (98%), Rat (99%), Zebrafish (86%), Xenopus laevis (89%), Chicken (90%), Rhesus Monkey (100%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The MCM3 protein is one of the highly conserved mini-chromosome maintenance proteins (MCM) involved in the initiation of eukaryotic genome replication. The hexameric MCM complex (MCM2–7) is a key component of the pre-replication complex (pre_RC) and plays a role in replication fork formation and recruitment of other DNA replication-related proteins.
MCM3 interacts directly with MCM5/CDC46 and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. This acetylation inhibits DNA replication initiation and cell cycle progression.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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