Thermo Fisher Scientific CD49a (Integrin alpha 1) Monoclonal Antibody (TS2/7), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

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Product Specifications

Additional Formats:

• PE
• Request custom conjugation

Product Specific Information

Description:
Monoclonal antibody TS2/7 reacts with human CD49a (integrin alpha 1). CD49a associates with CD29 (integrin beta 1) to form VLA-1, a type I transmembrane protein. This heterodimer is expressed by endothelial cells, activated T cells, monocytes, subsets of NK and memory T cells, smooth muscle cells, and mesenchymal stem cells. CD49a binds strongly to collagen IV and also interacts with collagen I, laminin, and semaphorin7A. It plays roles in cell survival, proliferation, homing, and inflammatory responses.

Applications Reported:
Validated for flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometry using stimulated normal human peripheral blood cells. Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with a blue laser (488 nm). Recommended filter: 710/50 bandpass (695/40 acceptable). Ensure instrument compatibility.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)

Storage up to 3 days in the dark at 4°C with minimal fluorescence loss. Clone-specific performance should be verified empirically.

Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Integrin alpha 1 (ITGA1) associates with beta 1 (ITGB1) to form a laminin/collagen receptor in neural and hematopoietic cells. ITGA1 contains a 206-amino acid I domain, three divalent cation-binding sites, a transmembrane domain, and 28 potential N-glycosylation sites. Expressed as a 180-kDa protein in fibroblast cells, ITGA1 is involved in osteoarthritic cartilage remodeling and regulates mesenchymal stem cell proliferation and cartilage production.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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