Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), PE-eFluor 610, eBioscience

Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), PE-eFluor 610, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 0.5 µg/test
• View 194 publications

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, Biotin
• Brilliant Violet 421, 605, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine, and cat FOXP3 (FORKHEAD BOX P3, SCURFIN, JM2).
FOXP3 is a 49–55 kDa transcriptional regulator identified as the defective gene in scurfy mice. High FOXP3 expression is characteristic of CD4+CD25+ regulatory T cells (Treg).

• Epitope mapping: amino acids 75–125 of mouse FOXP3
• Recommended buffer: Foxp3/Transcription Factor Buffer Staining Set (Product #00-5523-00)
• Application tested: Intracellular staining and flow cytometric analysis of mouse splenocytes
• Suggested protocol: BestProtocols® – Protocol B: One-step staining for intracellular (nuclear) proteins

Usage note: ≤0.5 µg/test in 100 µL final volume (10⁵–10⁸ cells/test). Optimize concentration for best performance.

Fluorochrome details:

• Excitation: 488–561 nm
• Emission: 607 nm
• Recommended filter: 610/20 band pass (PE-Texas Red® equivalent)
• Laser compatibility: Blue, Green, Yellow-Green lasers
• Light sensitivity: Protect vial and samples from light
• Fixation: Store up to 3 days at 4°C in dark using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

FOXP3 (Forkhead box protein 3)
FOXP3 is a transcriptional regulator crucial for immune homeostasis, expressed in CD25+CD4+ regulatory T cells. It is 381 amino acids long and absent in CD8+ T cells.
Defects in FOXP3 cause scurfy phenotype in mice and IPEX syndrome in humans (immune dysfunction, polyendocrinopathy, enteropathy, X-linked).
FOXP3 expression is regulated by p53 in response to DNA damage in human breast and colon cancer cells.

For Research Use Only. Not for use in diagnostic procedures.

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