Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2462)
인간 Ki-67 단백질을 인식하는 Mouse 모노클로날 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. 세포 증식 마커로 사용되며, PBS buffer에 보관 가능한 액상 형태입니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- 4288-MSM2-P1ABX
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2462)
Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2462)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MKI67, 2462 |
| Immunogen | Recombinant human Ki67 protein fragment (around aa 2293–2478) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly in late G1, S, G2, and M phases. It associates with euchromatin, centromeric heterochromatin, and the nucleolus through its forkhead-associated (FHA) domain.
Ki-67 shows cell cycle-dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase. It is widely used as a proliferation marker since it is absent in G0 cells and increases from G1 through mitosis.
In neoplastic tissues, Ki-67 index correlates strongly with tumor grade and is comparable to the thymidine-labelling index. It is routinely used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.