Thermo Fisher Scientific PRKAR2A Monoclonal Antibody (OTI1G7), TrueMAB
인간 PRKAR2A 단백질에 특이적인 Mouse IgG1 단일클론 항체. WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. 동결건조 형태로 제공되며 PBS buffer에 8% trehalose 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G7 |
| Immunogen | Full length human recombinant protein of human PRKAR2A produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
cAMP is a signaling molecule critical for various cellular functions. It activates cAMP-dependent protein kinase (AMPK), which transduces signals through phosphorylation of target proteins. The inactive holoenzyme of AMPK consists of two regulatory and two catalytic subunits. Upon activation, cAMP causes dissociation into regulatory dimers and free catalytic monomers.
The PRKAR2A gene encodes one of the regulatory subunits that can be phosphorylated by the catalytic subunit and may interact with A-kinase anchoring proteins, determining subcellular localization of AMPK.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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