
Thermo Fisher Scientific EEF1A1 Polyclonal Antibody
Human EEF1A1 단백질을 인식하는 Chicken IgY Polyclonal Antibody. Western blot, IHC, ELISA에 사용 가능. 고순도 액상 형태로 PBS buffer에 보존. 연구용 단백질 발현 및 면역학적 분석에 적합.
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Thermo Fisher Scientific EEF1A1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 5 µg/mL |
| Immunohistochemistry (IHC) | 1:100 |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.95 mg/mL |
| Purification | Selective precipitation |
| Storage Buffer | PBS, pH 7 |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Immunogen
Anti-EEF1A1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human EEF1A1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Antigen Information (OCR 추출 텍스트 통합):
- Target Family: EEF1A1
- UniProt ID: P68104-1
- NCBI Node ID: 9606
- Antigen Range: 1–462
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid freeze/thaw cycles.
- Centrifuge if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This affinity purified antibody is directed against human EEF1A1. It is an IgY fraction purified from monospecific chicken egg yolks by a multi-step process including selective precipitation, salt fractionation, and dialysis.
BLAST analysis suggests cross-reactivity with EEF1A1 from human, mouse, rat, bovine, rabbit, cat, horse, Chinese hamster, Gallus gallus, Pan troglodytes, and Pongo abelii. Possible cross-reactivity with EEF1A from Danio rerio and eef1as from Xenopus laevis.
Target Information
EEF1A1 protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
It forms a complex with PARP1 and TXK that acts as a Th1 cell-specific transcription factor, binding the promoter of IFN-gamma to regulate transcription and Th1 cytokine production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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