Thermo Fisher Scientific EEF1A1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

-

Immunohistochemistry (IHC)

1:100

-

ELISA (ELISA)

1:10,000-1:50,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Anti-EEF1A1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human EEF1A1 conjugated to Keyhole Limpet Hemocyanin (KLH). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EEF1A1,uniProtId:P68104-1,ncbiNodeId:9606,antigenRange:1,antigenLength:462,antigenImageFileName:200-901-MP6_EEF1A1_P68104-1_Chicken.svg,antigenImageFileNamePDP:200-901-MP6_EEF1A1_P68104-1_Chicken_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.95 mg/mL

Purification

selective precipitation

Storage buffer

PBS, pH 7

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human EEF1A1. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to EEF1A1 human, mouse, rat, bovine, rabbit, cat, horse, Chinese hamster, Gallus gallus, Pan troglodytes, and Pongoabelii. May have cross-reactivity to EEF1A to Danio rerio; and eef1as to Xenopuslaevis.

Target Information

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.