Thermo Fisher Scientific CRY2 Monoclonal Antibody (OTI1H5), TrueMAB

Applications and Tested Dilution

Product Specifications

Target Information

Various biochemical, physiological, and behavioral processes display circadian rhythms controlled by an internal biological clock. The central gears driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases.

In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors.

Mutant mice not expressing Cry1 or Cry2 display accelerated and delayed periodicity of locomotor activity, respectively. The combination of both proteins is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.