Thermo Fisher Scientific Nanog Polyclonal Antibody
Nanog 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot 및 IHC(P)에서 사용 가능. 인간 시료 반응성. 항원 친화 크로마토그래피로 정제. 배아줄기세포 연구 및 다능성 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the N-terminus of human Nanog (amino acids 29–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4 °C for short term. For long-term storage, store at −20 °C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Embryonic stem cells
- Cellular location: Nucleus
Target Information
NANOG (Nanog homeobox) is a divergent homeodomain protein that regulates pluripotency and differentiation in undifferentiated embryonic stem cells. NANOG mRNA is expressed in pluripotent mouse and human cell lines but absent in differentiated cells.
Human NANOG shares 52% overall amino acid identity with mouse NANOG and 85% identity within the homeodomain. It maps to gene locus 12p13.31 in humans and 6 F2 in mice.
Expression of NANOG is observed in the inner cell mass of the murine blastocyst. High NANOG expression is characteristic of undifferentiated embryonal carcinoma cell lines such as N-Tera. NANOG functions as a transcriptional regulator involved in proliferation and self-renewal of embryonic stem (ES) cells.
The role of NANOG in embryonic development suggests its potential utility in generating stem cells for regenerative medicine. Induced pluripotent stem (iPS) cells can be created by expressing transcription factors such as NANOG, Sox2, Klf4, and c-Myc, enabling reprogramming without viral vectors. Overexpression of NANOG promotes S-phase entry and proliferation.
Diseases associated with NANOG dysfunction include teratocarcinoma and germ cell/embryonal cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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