Thermo Fisher Scientific ERCC1 Monoclonal Antibody (OTI1C6), TrueMAB
ERCC1 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC, ICC, Flow Cytometry에 적합합니다. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공되며, DNA 손상 복구 연구에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C6 |
| Immunogen | Full length human recombinant ERCC1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, an additional desalting step is recommended (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5′-incision during DNA repair.
In conjunction with SLX4, it plays a key role in the repair of interstrand cross-links (ICL) and participates in processing anaphase bridge-generating DNA structures.
ERCC1 is also required for homology-directed repair (HDR) of DNA double-strand breaks, working together with SLX4.
(Source: UniProt)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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