Thermo Fisher Scientific CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), PerCP-eFluor 710, eBioscience
마우스 GITR(TNFRSF18)을 인식하는 DTA-1 단클론 항체로, PerCP-eFluor 710 형광표지되어 있습니다. Flow Cytometry에 적합하며, 0.004 µg/test 농도로 사용 가능합니다. Treg 세포 연구 및 면역조절 기전 분석에 유용합니다.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.004 µg/test
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DTA-1 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573783 |
Additional Available Formats
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The DTA-1 monoclonal antibody reacts with mouse GITR (Glucocorticoid-Induced TNFR family gene, TNFRSF18).
In naive mice, GITR is predominantly expressed by CD4+CD25+ regulatory T cells (Treg) and CD25+ CD4+ CD8− thymocytes.
Stimulation of GITR with DTA-1 antibody abrogates Treg-mediated suppression. Although it does not deplete CD357-expressing cells in vivo, it can induce tumorogenesis. Removal of GITR-expressing Treg cells or DTA-1 administration may lead to organ-specific autoimmune disease.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.004 µg/test (100 µL final volume; 10⁵–10⁸ cells/test). Optimal titration recommended.
Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with the 488 nm blue laser.
Recommended filter: 710/50 bandpass (695/40 acceptable alternative).
Ensure instrument compatibility.
Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:
Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Target: Tumor necrosis factor receptor superfamily member 18 (TNFRSF18, also known as GITR or AITR)
- Encoded by the TNFRSF18 gene (mapped to 1p36.33)
- Member of the TNF-receptor superfamily
- Expression increases upon T-cell activation
- Plays a key role in immunological self-tolerance maintained by CD25⁺CD4⁺ Treg cells
- Knockout studies suggest involvement in CD3-driven T-cell activation and programmed cell death
- Three alternatively spliced transcript variants reported
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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