Thermo Fisher Scientific CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.004 µg/test
• Publications: View 1 publication

Product Specifications

Additional Available Formats

• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The DTA-1 monoclonal antibody reacts with mouse GITR (Glucocorticoid-Induced TNFR family gene, TNFRSF18).
In naive mice, GITR is predominantly expressed by CD4+CD25+ regulatory T cells (Treg) and CD25+ CD4+ CD8− thymocytes.
Stimulation of GITR with DTA-1 antibody abrogates Treg-mediated suppression. Although it does not deplete CD357-expressing cells in vivo, it can induce tumorogenesis. Removal of GITR-expressing Treg cells or DTA-1 administration may lead to organ-specific autoimmune disease.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.004 µg/test (100 µL final volume; 10⁵–10⁸ cells/test). Optimal titration recommended.

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with the 488 nm blue laser.
Recommended filter: 710/50 bandpass (695/40 acceptable alternative).
Ensure instrument compatibility.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)

Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Target: Tumor necrosis factor receptor superfamily member 18 (TNFRSF18, also known as GITR or AITR)

• Encoded by the TNFRSF18 gene (mapped to 1p36.33)
• Member of the TNF-receptor superfamily
• Expression increases upon T-cell activation
• Plays a key role in immunological self-tolerance maintained by CD25⁺CD4⁺ Treg cells
• Knockout studies suggest involvement in CD3-driven T-cell activation and programmed cell death
• Three alternatively spliced transcript variants reported

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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