Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 488
DyLight 488 형광 표지를 가진 염소 유래 항-마우스 IgG(H+L) 2차 항체로, WB, IHC, ICC, Flow 등 다양한 면역실험에 적합. 교차흡착 처리로 비특이적 반응 최소화. 492/519 nm 형광 특성. 항원 친화 크로마토그래피로 정제됨.
- 카탈로그번호
- 35503
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C (protected from light) |
| Shipping Conditions | Ambient |
| RRID | AB_1965946 |
| Target | IgG |
| Cross Adsorption | Against human, bovine, horse, rabbit, swine, goat, and rat serum proteins |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #35503 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
It reacts with the heavy chains of mouse IgG and the light chains common to most mouse immunoglobulins, but does not react with non-immunoglobulin serum proteins.
This antibody is solid-phase adsorbed to ensure minimal cross-reactivity with human, bovine, horse, rabbit, swine, goat, and rat serum proteins, though minor cross-reactivity with other species may occur.
Store protected from light at 4°C.
For extended storage, add an equal volume of glycerol (final 50%) and store at -20°C.
DyLight 488 Amax = 493 nm; Emax = 518 nm.
Mole Dye/Mole Protein Ratio is lot-dependent.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of target proteins and enable versatile detection systems (e.g., HRP, AP, fluorescence).
Secondary antibodies provide enhanced sensitivity through signal amplification, as multiple secondary antibodies can bind to one primary antibody.
Typically, these antibodies are generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or conjugated for specific applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Available Conjugates
A wide range of conjugates are available for Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, including:
- Agarose
- Alexa Fluor Series (350, 405, 430, 488, 514, 532, 546, 555, 568, 594, 633, 635, 647, 660, 680, 700, 750, 790)
- AP, APC, APC-Alexa Fluor 750
- Biotin, Biotin-XX
- BODIPY FL
- Cyanine3, Cyanine5
- DyLight Series (350, 405, 550, 594, 633, 650, 680, 755, 800)
- FITC, HRP, Pacific Blue, PE, PE-Alexa Fluor 610, Rhodamine, Rhodamine Red-X, Texas Red-X, TRITC
- Custom conjugation available upon request.
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