Thermo Fisher Scientific Atg16L1 Polyclonal Antibody
ATG16L1 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체. Western blot 및 면역침강(IP)에 적합하며, 인간 시료 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 2–10 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 50 and 100 of human ATG16 Autophagy Related 16-like 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
Autophagy related protein 16L is encoded by the ATG16L gene, located on chromosome 2 in humans. It is also known as WD30 or IBD10 (Inflammatory Bowel Disease 10).
ATG12 and ATG5 conjugate with each other and undergo homo-oligomerisation with ATG16L to form an 800 kDa Atg16L complex. This complex facilitates coupling or lipidation of LC3 to phosphatidylethanolamine (PE), a widely used marker for autophagy.
The ATG16L complex, along with other ATGs such as ATG18 and ATG21, prevents premature cleavage of ATG8-PE by the cysteine protease Atg4 until the autophagosome is complete.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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