Thermo Fisher Scientific GeneChip Porcine Gene 1.0 ST Array, 30 arrays

Applied Biosystems™ GeneChip™ Porcine Gene 1.0 ST Array

The GeneChip™ Porcine Gene 1.0 ST Arrays enable you to:

• Measure expression across the entire gene with higher resolution and accuracy than classical 3’-biased microarray solutions
• Obtain accurate and reproducible data using multiple independent measurements for each transcript

Background

Model and applied research organisms play a vital role in comparative genomics, evolutionary biology, and disease mechanism studies. The GeneChip Gene 1.0 ST Arrays enable whole-transcript coverage for a wide range of model and applied organisms.
These arrays were designed in collaboration with leading researchers, including:

• Alan Archibald, Roslin Institute (Porcine Array design)
• Leonard Zon, Harvard Medical School (Zebrafish Array design)
• Yi Zhou, Children’s Hospital Boston

Key Benefits

• Highest transcript coverage: Up to 26 probes per transcript for confident expression measurement
• Whole-transcriptome analysis: Detect transcript isoforms missed by 3’-biased arrays
• High data correlation: Inter- and intra-array signal correlation (R > 0.99)

Proven Performance

GeneChip Gene 1.0 ST Arrays deliver whole-transcriptome coverage based on the latest genomic content.
This design ensures:

• Accurate detection across the full gene length
• Identification of multiple transcript isoforms (splice variants, non-polyadenylated transcripts, alternative polyadenylation, truncated transcripts)
• Higher resolution and accuracy compared to classical 3’-biased designs

Complete Reagents, Software and Instrumentation Solution

For optimal performance, Gene 1.0 ST Arrays are supported by a complete system including:

• GeneChip WT Sense Target Labeling and Control Reagents – for assay preparation
• GeneChip Fluidics Station 450 – automated array processing for reproducibility
• GeneChip Scanner 3000 7G – high-quality image acquisition

Data analysis tools include:

• GeneChip Expression Console Software – easy probe summarization and quality evaluation
• NetAffx Analysis Center – comprehensive probe annotations and sequence information
• Integrated Genome Browser (IGB) – visualization of genomic context and annotations

Workflow

Data analysis workflow is similar to 3’ expression arrays, utilizing:

• Expression Console 1.1
• GeneChip-compatible third-party software
• NetAffx Analysis Center and IGB for annotation and visualization

Content Profile

GeneChip Gene 1.0 ST Arrays provide comprehensive coverage of the transcribed genome:

• Up to 26 unique 25-mer probes per transcript, interrogating up to 650 bases
• High probe coverage ensures superior performance and confidence
• Enables data updates as genomic knowledge evolves

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