Thermo Fisher Scientific VeriFiler Express PCR Amplification Kit

Applied Biosystems™ VeriFiler™ Express PCR Amplification Kit

The VeriFiler Express PCR Amplification Kit is a direct-amplification STR kit that combines high discrimination power with superior performance and fast time-to-results. It enables multiplex amplification of 22 STR loci (as in the GlobalFiler Express kit), plus the highly discriminating Penta D and Penta E loci and two gender markers (Amelogenin, Yindel) for maximum concordance and confidence in paternity and relationship testing.

Powered by breakthrough 6-dye technology, the kit provides optimal performance and excellent discrimination power to obtain accurate results for single-source samples in less time.

Key Features

• Direct amplification from common paternity testing samples without extraction or purification:
  • Buccal samples on treated/untreated paper
  • Buccal samples on cotton swab
  • Blood samples on treated/untreated paper
• Accelerates time-to-result with minimal data quality reduction
• Supports high-throughput and automated workflows
• Improves genotyping accuracy with superior primer design
• Enhances accuracy through expanded allelic ladder and virtual bins

High First-Pass Success Rates

The kit enables high-quality direct PCR amplification with improved first-pass success rate:

• Well-resolved peaks above background noise
• Minimal allelic drop-out, off-ladder allele calls, and off-scale data
• Balanced profile within each dye color for maximized Intracolor Balance (ICB) values

Maximizes Concordance

Optimized primers ensure concordance with GlobalFiler Express, NGM Select, Identifiler, and VeriFiler Direct kits:

• Simplified comparison with existing datasets
• Reduced need for repeat analyses
• Similar configuration for easy training and implementation

Complete Validated Solution

Part of a comprehensively validated system for STR-based human identification, including:

• DNA quantification kits, STR kits, instrumentation, software, and consumables
• Developed and validated with Applied Biosystems instruments and software
• Compliant with SWGDAM and DNA Databasing Laboratory Quality Assurance Standards

Each lab should perform its own internal validation studies before routine use.

Commitment to Quality

Manufactured under ISO 9001 and ISO 18385 quality systems.
Each lot includes a Certificate of Analysis confirming quality assurance compliance.

Experience Matters

Thermo Fisher Scientific uniquely designs and validates reagents, instruments, and data analysis software as an integrated DNA testing system.
Technical service scientists and field specialists provide expert support for assay optimization and troubleshooting.

For licensing and limited use restrictions, visit:
www.thermofisher.com/HIDlicensing

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