Thermo Fisher Scientific TDG Monoclonal Antibody (GT311)
TDG 단백질을 인식하는 GT311 클론의 Mouse monoclonal antibody. Western blot 및 IHC(Paraffin)에서 검증됨. Human과 Mouse에 반응하며, Protein A로 정제된 1 mg/mL 액상 제품. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT311 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human TDG |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787152 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (99%), Xenopus laevis (92%), Chicken (95%), Bovine (99%).
- Positive control: human TDG-transfected 293T cells.
- Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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