Thermo Fisher Scientific GATA3 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Suggested positive control: human brain tissue lysate
• PA5-20892 can be used with blocking peptide PEP-1006

Target Information

The genes for all four subunits of the T-cell antigen receptor (alpha, beta, gamma, and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all four TCR genes.
GATA3 was previously shown in chickens to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was detected in T cells but not in B cells or macrophages, indicating its abundant expression in the T-lymphocyte lineage. It is thought to participate in T-cell receptor gene activation through enhancer binding.
Labastie et al. (1994) cloned the human gene and the 5′ end of the mouse gene. The human gene comprises six exons distributed over 17 kb of DNA. Its two zinc fingers are encoded by separate exons highly conserved with those of GATA1, but no other structural homologies between the two genes were found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.