Thermo Fisher Scientific LXR alpha Polyclonal Antibody
LXR alpha 단백질을 특이적으로 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot 및 ICC/IF에 적합하며, 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 고품질 액상 항체로 연구용으로 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 4 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 | View 1 publication |
| Gel Shift (GS) | – | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues C D(318) F S Y N R E D F A K A(329) of human LXR alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325600 |
Product Specific Information
PA1-330 detects recombinant human liver X receptor (LXR) alpha and does not detect human recombinant LXR beta protein.
PA1-330 has been successfully used in ICC/IF and Western blot procedures. By Western blot, this antibody detects an ~64 kDa protein representing recombinant human LXR alpha.
The PA1-330 immunogen is a synthetic peptide corresponding to residues C D(318) F S Y N R E D F A K A(329) of human LXR alpha.
The PA1-330 immunizing peptide (Cat. # PEP-114) is available for use in neutralization and control experiments.
Target Information
NR1H3 (oxysterols receptor LXR-alpha) is a nuclear receptor that exhibits ligand-dependent transcriptional activation activity. Its interaction with retinoic acid receptor (RXR) shifts RXR from a silent DNA-binding partner to an active ligand-binding subunit, mediating retinoid responses through LXRES target genes.
LXRES are DR4-type response elements characterized by direct repeats of two similar hexanucleotide half-sites spaced by four nucleotides.
NR1H3 plays an important role in regulating cholesterol homeostasis, including MYLIP-dependent ubiquitination of LDLR, VLDLR, and LRP8. It also interacts functionally with RORA for regulation of liver metabolism genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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