Thermo Fisher Scientific LXR alpha Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Gel Shift (GS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C D(318) F S Y N R E D F A K A(329) of human LXR alpha. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LXR alpha,uniProtId:Q13133-1,ncbiNodeId:9606,antigenRange:318-329,antigenLength:447,antigenImageFileName:PA1-330_LXR_alpha_Q13133-1_Rabbit.svg,antigenImageFileNamePDP:PA1-330_LXR_alpha_Q13133-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325600

Product Specific Information

PA1-330 detects recombinant human liver X receptor (LXR) alpha. This antibody does not detect human recombinant LXR beta protein.

PA1-330 has been successfully used in ICC/IF and Western blot procedures. By Western blot, this antibody detects an ~64 kDa protein representing recombinant human LXR alpha.

The PA1-330 immunogen is a synthetic peptide corresponding to residues C D(318) F S Y N R E D F A K A(329) of human LXR alpha. The PA1-330 immunizing peptide (Cat. # PEP-114) is available for use in neutralization and control experiments.

Target Information

NR1H3 (oxysterols receptor LXR-alpha) is a nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Its interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanucleotide half-sites spaced by four nucleotides. NR1H3 plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR, and LRP8. It also interplays functionally with RORA for the regulation of genes involves in liver metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.