Thermo Fisher Scientific DR3 Monoclonal Antibody (JD3), PE, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.125 µg)/test
• View 1 publication

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• Request custom conjugation

Product Specific Information

The JD3 monoclonal antibody reacts with human DR3, also known as Apo-3, WSL-1, TRAMP, LARD, DDR3, and TR3.
DR3 is a death receptor expressed at low levels by monocytes and granulocytes. Interaction of DR3 with its ligand activates the NF-kappa B pathway and induces apoptosis. Initially thought to be a receptor for TWEAK, later studies revealed TWEAK induces apoptosis via receptors distinct from DR3.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested by flow cytometry on normal human peripheral blood cells and DR3-transfected cells
• Recommended Use: 5 µL (0.125 µg) per test (in 100 µL final volume, 10⁵–10⁸ cells/test)
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser: Blue, Green, Yellow-Green Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

DR3/TNFRSF25 is a member of the TNF-receptor superfamily, expressed preferentially in lymphocyte-rich tissues. It plays a role in regulating lymphocyte homeostasis and can stimulate NF-kappa B activity and apoptosis. Signal transduction is mediated by death domain-containing adaptor proteins.
Mouse knockout studies indicate DR3/TNFRSF25 is involved in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants exist, many encoding secreted isoforms. In B and T cells, activation induces expression of full-length, membrane-bound isoforms that control lymphocyte proliferation.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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