Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Yellow 690, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Yellow 690, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

Unconjugated, Alexa Fluor 488, 532, 561, 660, 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet series, eFluor series, FITC, PE series, Super Bright series, and NovaFluor variants (Blue, Red, UV, Violet, Yellow).
Custom conjugation available upon request.

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as either CD8αβ heterodimer or CD8αα homodimer.
CD8 binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a role in T cell development and activation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported

• Flow cytometric analysis of mouse splenocytes.

Applications Tested

• Tested by flow cytometry; use ≤ 0.5 µg/test (100 µL final volume).
• Cell number range: 10⁵–10⁸ cells/test.
• Antibody should be titrated for optimal performance.

Important Notes

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
  • Add 5 µL per sample (regardless of number of NovaFluors).
  • For single-color controls, use 5 µL per 100 µL cell sample.

NovaFluor Technology

NovaFluor conjugates are based on Phiton™ technology, using nucleic acid dye structures for engineered fluorescent signatures minimizing spillover and spread.

• Excitation: 552 nm
• Emission: 690 nm
• Laser: 561 nm (Yellow)

Note: Internal testing shows non-specific B cell staining in SJL mice; not observed in BALB/c or C57BL/6 mice.

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein involved in signal transduction, T cell differentiation, and activation.
Expressed as a CD8αβ heterodimer on T cells and CD8αα homodimer on subsets of T cells, NK cells, monocytes, and dendritic cells.
Functions as a co-receptor for MHC class I molecules, recruiting Lck kinase upon ligation, leading to CTL activation and pathogen/tumor clearance.
Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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