Thermo Fisher Scientific EIF4E Monoclonal Antibody (OTI6C3), TrueMAB
EIF4E 단백질을 인식하는 Mouse Monoclonal Antibody(OTI6C3)로, WB, IHC(P), Flow Cytometry에 사용 가능. Human 시료 반응성. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 | - |
| Flow Cytometry (Flow) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6C3 |
| Immunogen | Full length human recombinant protein of human EIF4E produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
eIF4E is involved in multiple cellular processes such as translational efficiency, splicing, mRNA stability, and RNA nuclear export.
All eukaryotic mRNAs contain a 7-methylguanosine cap (m7GpppX) at the 5′ end. EIF4E acts as a eukaryotic translation initiation factor that directs ribosomes to this cap structure.
It is a 24-kDa polypeptide that exists both freely and as part of the EIF4F complex. EIF4E is the rate-limiting component in eukaryotic translation initiation and mediates mRNA-ribosome binding.
The EIF4F complex also includes EIF4A (50 kDa, ATPase and RNA helicase activity) and EIF4G (220 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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