Thermo Fisher Scientific CENPF Monoclonal Antibody (14C10 1D8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

14C10 1D8

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 1759-2093. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CENPF,uniProtId:P49454-1,ncbiNodeId:9606,antigenRange:1759-2093,antigenLength:3114,antigenImageFileName:MA1-23185_CENPF_P49454-1_House_mouse.svg,antigenImageFileNamePDP:MA1-23185_CENPF_P49454-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560120

Product Specific Information

Recommended positive controls: HeLa (G2 phase).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitosis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.