Thermo Fisher Scientific GCK Polyclonal Antibody
Thermo Fisher Scientific의 GCK Polyclonal Antibody는 인간, 마우스, 랫트 시료에서 Glucokinase 단백질을 검출하기 위한 항체입니다. Western blot에 적합하며, 고순도의 Rabbit IgG 폴리클로날 항체로 PBS/glycerol 버퍼에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 250–465 of human Glucokinase (GCK) (NP_000153.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.78 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805957 |
Product Specific Information
Immunogen sequence:
RMCVNTEWGA FGDSGELDEF LLEYDRLVDE SSANPGQQLY EKLIGGKYMG ELVRLVLLRL VDENLLFHGE ASEQLRTRGA FETRFVSQVE SDTGDRKQIY NILSTLGLRP STTDCDIVRR ACESVSTRAA HMCSAGLAGV INRMRESRSE DVMRITVGVD GSVYKLHPSF KERFHASVRR LTPSCEITFI ESEEGSGRGA ALVSAVACKK ACMLGQ
Positive Samples: LO2, A-549, mouse liver, rat liver
Cellular Location: Cytoplasm, Nucleus
Target Information
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase: one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. Unlike other hexokinases, this enzyme is not inhibited by glucose-6-phosphate and remains active when glucose is abundant. Mutations in this gene are associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young type 2 (MODY2), and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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