Thermo Fisher Scientific CD59 Recombinant Rabbit Monoclonal Antibody (029)

Applications

Flow Cytometry (Flow)

• Tested Dilution: 1:25–1:100

ELISA

• Tested Dilution: 1:5,000–1:10,000

Product Specifications

Additional Formats

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to avoid contamination.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

This antibody has specificity for Human CD59.

Target Information

CD59 (Protectin) is a small (18–20 kDa) GPI-anchored, ubiquitously expressed inhibitor of the membrane attack complex (MAC). It regulates complement cascade by preventing autologous cell lysis through inhibition of cytolytic pore formation.
CD59 binds complement C8 and/or C9 during MAC assembly, blocking multiple C9 incorporations required for pore formation. It also acts as a low-affinity ligand of human CD2, contributing to T cell costimulation and lymphocyte signal transduction.
Mutations in CD59 cause CD59 deficiency, associated with hemolytic anemia, thrombosis, and cerebral infarction. Multiple alternatively spliced transcript variants are known.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.