Thermo Fisher Scientific GPAM Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 594-828 of human GPAM (NP_0012318781) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GPAM,uniProtId:Q9HCL2-1,ncbiNodeId:9606,antigenRange:594-828,antigenLength:828,antigenImageFileName:PA5-93092_GPAM_Q9HCL2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-93092_GPAM_Q9HCL2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.45 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806619

Product Specific Information

Immunogen sequence: LNKRGLGGPT STPPNLISQE QLVRKAASLC YLLSNEGTIS LPCQTFYQVC HETVGKFIQY GILTVAEHDD QEDISPSLAE QQWDKKLPEP LSWRSDEEDE DSDFGEEQRD CYLKVSQSKE HQQFITFLQR LLGPLLEAYS SAAIFVHNFS GPVPEPEYLQ KLHKYLITRT ERNVAVYAES ATYCLVKNAV KMFKDIGVFK ETKQKRVSVL ELSSTFLPQC NRQKLLEYIL SFVVL; Positive Samples: Mouse heart, Mouse lung, Rat brain; Cellular Location: Mitochondrion outer membrane, Multi-pass membrane protein

Target Information

Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets. At least two isoforms of GPAT1 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.