Thermo Fisher Scientific Phospho-alpha Adducin (Ser436) Polyclonal Antibody
Phospho-alpha Adducin (Ser436) 폴리클로날 항체로, 인간·생쥐·랫트 반응성. WB, IHC, ICC, ELISA 등 다양한 응용 가능. Protein A 정제, 1 mg/mL 농도, -20°C 보관. 세포골격 단백질 연구용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of Ser436 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein composed of related subunits produced from distinct genes but sharing similar structure.
Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region.
Alpha- and gamma-adducins are ubiquitously expressed, while beta-adducin is highly expressed in brain and hematopoietic tissues.
Adducin binds strongly to Ca(2+)/calmodulin and serves as a substrate for protein kinases A and C.
Alternative splicing leads to multiple variants encoding distinct isoforms, though not all have been fully described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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