Thermo Fisher Scientific Zika Virus NS1 (strain Zika SPH2015) Monoclonal Antibody (15)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

ELISA (ELISA)

1:1,000-1:2,000

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

15

Immunogen

Recombinant Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2785803

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1.

Target Information

As a member of the Flaviviridae family, ZIKV has a single positive sense RNA genome, which is initially translated as a single polyprotein which is then cleaved post-transitionally into three structural proteins (C, PrM or M, and E) and seven nonstructural (NS) proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5)(1). The glycosated NS1 forms a homodimer inside the cells and is necessary for viral replication and late in infection (2). NS1 is also secreted into the extracellular space as a hexameric lipoprotein particle, which assists the virus in immune evasion and pathogenesis by interacting with components from both innate and adaptive immune systems as well as other host factors (1). Reference 1) Song, H.; Qi, J.; Haywood, J.; Shi, Y.; Gao, G.F. (2016). Nat. Struct. Mol. Biol. 23, 456-458. 2) Suthar, M.S., Diamond, M.S. & Gale, M. Jr. (2013). Nat. Rev. Microbiol. 11, 115-128.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.