Thermo Fisher Scientific SMAD5 Monoclonal Antibody (3A10)
SMAD5 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 3A10). Western blot, ICC/IF, ELISA에 적합하며 인간 시료에 반응. PBS buffer, 무보존제, -20°C 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific SMAD5 Monoclonal Antibody (3A10)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.3 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3A10 |
| Immunogen | SMAD5 (AAH09682, 105 a.a. approximately 182 a.a) partial recombinant protein with GST tag (MW of GST tag alone: 26 KDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
KPLDICEFPF GSKQKEVCIN PYHYKRVESP VLPPVLVPRH NEFNPQHSLL VQFRNLSHNE PHMPQNATFP DSFHQPNN
Target Information
SMADs are members of the MAD-related family of molecules. These intracellular proteins are essential components in the signaling pathways of the serine/threonine kinase receptors of the TGF-beta superfamily.
SMADs can be categorized as:
- Receptor-regulated SMADs (R-SMADs): SMAD1, 2, 5, 8, and 9
- Common-mediator SMAD (Co-SMAD): SMAD4
- Inhibitory SMADs (I-SMADs): SMAD6 and 7
SMAD1, 5, 8, and 9 share high sequence homology, and antibodies may recognize common regions among them. SMAD8 and SMAD9 are often used interchangeably in literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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