Thermo Fisher Scientific UBE2M Monoclonal Antibody (OTI2D9), TrueMAB
UBE2M 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 면역세포염색에 적합. HEK293T 세포에서 발현된 full-length human UBE2M 단백질로 면역화. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2D9 |
| Immunogen | Full length human recombinant protein of human UBE2M produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Protein ubiquitination is a key cellular mechanism for degrading abnormal or short-lived proteins. This process involves three enzyme classes:
- E1 (ubiquitin-activating enzymes)
- E2 (ubiquitin-conjugating enzymes)
- E3 (ubiquitin-protein ligases)
UBE2M encodes an E2 ubiquitin-conjugating enzyme that interacts with the ubiquitin-like protein NEDD8, which can be conjugated to cellular proteins such as Cdc53/cullin.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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